Protective Effect of Curcumin on Lipopolysaccharide-Induced Rat Cardiomyocyte Hypertrophy

To study the protective effect of curcumin on lipopolysaccharide-induced rat cardiomyocyte hypertrophy is main objective study. Cardiomyocytes were purified and cultured by differential adherence in vitro then they divided into control group, lipopolysaccharide lipopolysaccharide+curcumin low-dose (12.5 mg/l) medium-dose (25 group high-dose (50 group. The morphological changes observed under a phase-contrast inverted microscope cell volume was measured. total protein content detected Coomassie brilliant blue kit. Enzyme-linked immunosorbent assay method used to detect level tumor necrosis factor alpha. TILL imaging system observe intracellular calcium ions. Compared with significantly increased volume, content, alpha (p<0.01) instantaneous peak 12.5, 25, 50 mg/l treatment inhibited increase ions induced showed dose-dependent effect. Curcumin has lipopolysaccharides, which may be related inhibition production overload.